Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC), is a very popular analytical tool for characterizing natural and synthetic polymers. Advanced GPC/SEC analysis using multi-detectors is increasingly being used to measure absolute molecular weight independent of column retention volume and to add other measurements such as those of intrinsic viscosity and hydrodynamic radius. With this information, scientists can accurately study how changes in molecular weight and structure affect polymer performance.
Advances in detector technology have enabled more accurate and information-rich measurements, but the quality of those measurements is still dependent on the quality of the separation, which itself remains dependent on the column. Good resolution depends on matching the column pore size to the sample of interest and an ideal separation requires no interaction between the sample and the column material. Additionally, in order to maximize light scattering signal-to-noise, columns should be stable over time and not shed any particulates, to which the light scattering detector will be very sensitive.
From the detector standpoint, inter-detector distances should be reduced to minimize peak dispersion. This webinar will discuss how high quality columns and detectors both contribute to high quality GPC/SEC measurements. We will also discuss how the two interact with each other and show data to demonstrate some ideal measurements as well as showing some of the symptoms of poor measurements while discussing the possible causes.
X-ray reflectometry (XRR) is a well-established analytical method for the characterization of thin layered structures, surfaces and interfaces. It is used to determine layer thicknesses and densities and provides roughness-related information. The basics of XRR and the analysis of XRR data were discussed in one of our webinars last year. This time, the focus will be on a typical workflow, from setting up the X-ray optics of a diffractometer, the essential steps of the sample alignment procedures to the final XRR measurement.
Don’t miss the useful practices and tips that we will share with you in this webinar. They will help you to collect high-quality XRR data from your layered samples.